Laser assisted zona hatching: What’s the evidence to justify its use?

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Implantation failures: success of assisted hatching with quarter-laser zona thinning.

Implantation failure after IVF is one of the factors associated with a reduced chance of pregnancy for some patients. Assisted hatching methodologies are designed to facilitate the embryo's escape from the zona pellucida, and this strategy has been suggested as a means of improving pregnancy rates in patients with previous implantation failure. The aim of this prospective and randomized study w...

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The Use of Laser-assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate

The Use of Laser-Assisted Hatching in Bovine in Vitro Produced Embryos to Improve Pregnancy Rate. (August 2008) Suzanne Lynn Menges, B.S., Texas A&M University Chair of Advisory Committee: Dr. Duane Kraemer In vitro produced (IVP) embryos not hatching from the zona pellucida (ZP) after transfer is one possible contributing factor of a lower pregnancy rate when compared to in vivo embryos. This ...

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Laser-assisted zona pellucida thinning prior to routine ICSI.

BACKGROUND In MII oocytes showing difficult oolemma breakage, ICSI can cause an increase in the degeneration rate. This may be overcome by laser-assisted ICSI using a 5-10 micro m opening in the zona pellucida for injection. However, such a small opening might impair the hatching process, especially if assisted hatching is applied in addition. In order to prevent this, the present study used ro...

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Laser-assisted zona pellucida thinning does not facilitate hatching and may disrupt the in vitro hatching process: a morphokinetic study in the mouse

STUDY QUESTION Does laser-assisted zona thinning of cleavage stage mouse embryos facilitate hatching in vitro? SUMMARY ANSWER No, unlike laser zona opening, zona thinning does not facilitate embryo hatching. WHAT IS KNOWN ALREADY Artificial opening of the zona pellucida facilitates hatching of mouse and human embryos. Laser-assisted zona thinning has also been used for the purpose of assist...

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Effect of laser-assisted multi-point zona thinning on development and hatching of cleavage embryos in mice

OBJECTIVE This study aimed to examine the effect of laser-assisted zona thinning (LAZT) at one or four-points on the blastocyst formation and hatching process in mice with respect to female age. METHODS Eight-cell or morula embryos collected from superovulated C57BL female mice with different ages (6-11 and 28-31 weeks) were treated with LAZT at one-point (LAZT1) or four-points (LAZT4). The z...

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ژورنال

عنوان ژورنال: Middle East Fertility Society Journal

سال: 2011

ISSN: 1110-5690

DOI: 10.1016/j.mefs.2011.03.001